翻訳と辞書 Words near each other ・ End-diastolic volume ・ End-Ediacaran extinction ・ End-group ・ End-of-file ・ End-of-history illusion ・ End-of-life ・ End-of-life (product) ・ End-of-life care ・ End-of-text character ・ End-of-the-day betting effect ・ End-of-train device ・ End-of-transmission character ・ End-of-year rugby union internationals ・ End-on-end ・ End-plate potential ・ End-sequence profiling ・ End-stopping ・ End-systolic volume ・ End-Time Illusion ・ End-to-end ・ End-to-end auditable voting systems ・ End-to-end delay ・ End-to-end encryption ・ End-to-end principle ・ End-to-end vector ・ End-use certificate ・ End-user (disambiguation) ・ End-user certificate ・ End-user computing ・ End-user development Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク End-sequence profiling ： ウィキペディア英語版 End-sequence profiling End-sequence profiling (ESP) (sometimes “Paired-end mapping (PEM)”) is a method based on sequence-tagged connectors developed to facilitate ''de novo'' genome sequencing to identify high-resolution copy number and structural aberration such as inversion and translocation. Briefly, the target genomic DNA is isolated and partially digested with restriction enzymes into large fragments. Following size-fractionation, the fragments is cloned into plasmids to construct artificial chromosome such as bacterial artificial chromosomes (BAC) which will be sequenced and compared to the reference genome. The differences, including orientation and length variations between constructed chromosomes and the reference genome, will suggest copy number and structural aberration. == Artificial chromosome construction == Before analyzing target genome structural aberration and copy number variation (CNV) with ESP, the target genome is usually amplified and conserved with artificial chromosome construction. The classic strategy to construct an artificial chromosome is bacterial artificial chromosome (BAC). Basically, the target chromosome is randomly digested and inserted into plasmids which are transformed and cloned in bacteria. The size of fragments inserted is 150–350 kb. Another commonly used artificial chromosome is fosmid. The difference between BAC and fosmids is the size of the DNA inserted. Fosmids can only hold 40 kb DNA fragments, which allows a more accurate breakpoint determination. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「End-sequence profiling」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.